{"created":"2023-05-15T08:14:28.698334+00:00","id":504,"links":{},"metadata":{"_buckets":{"deposit":"d9e342ca-5369-41af-83fe-ee1acd463b7c"},"_deposit":{"created_by":8,"id":"504","owners":[8],"pid":{"revision_id":0,"type":"depid","value":"504"},"status":"published"},"_oai":{"id":"oai:shokei.repo.nii.ac.jp:00000504","sets":["1:5:49"]},"author_link":["1446","1447"],"item_2_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-07-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"79","bibliographicNumberOfPages":"12","bibliographicPageEnd":"80","bibliographicPageStart":"69","bibliographic_titles":[{"bibliographic_title":"尚絅学院大学紀要","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of Shokei Gakuin University","bibliographic_titleLang":"en"}]}]},"item_2_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"ビタミンEの8種の同族体（α-、β-、γ-、δ-トコトリエノールおよびトコフェロール）の逆相HPLCによる同時分析法の確立を目的に、分離カラムおよび分離温度を中心に検討した。その結果、C30カラムを用い低温条件を用いることにより、8種の同族体の完全分離を達成することができた。一方、低温条件でのカラム効率の低下については、流速を下げることにより改善することができ、最終的に、カラムとしてDevelosil C30-UG-3（4.6 x 150mm）、移動相としてメタノール（100%）を用い、流速0.9mL/min、-4℃で溶出し、蛍光検出（Ex. 297nm、Em. 325nm）することにより、ビタミンEの8種の同族体を20分以内で分離分析することができた。検量線はsub ng ～ 10ngオーダーの範囲で良好な直線となり、S/N=3における検出限界は、0.011 ～ 0.055ngであった。実際の食用油において、トコトリエノール類の分析に共存する成分による影響が認められたが、ODSカラムとC30カラムを組み合わせた2次元HPLC法により共存する成分の影響を排除することができた。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_2_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"17","subitem_description_type":"Other"}]},"item_2_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P","subitem_description_type":"Other"}]},"item_2_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"論文","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_2_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24511/00000484","subitem_identifier_reg_type":"JaLC"}]},"item_2_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA12012046","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2433507X","subitem_source_identifier_type":"ISSN"}]},"item_2_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"尚絅学院大学総合人間科学系健康栄養部門"}]},"item_2_text_7":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Shokei Gakuin University"}]},"item_2_title_3":{"attribute_name":"論文名よみ","attribute_value_mlt":[{"subitem_title":"ギャクソウ HPLC ホウ ニ ヨル トコトリエノール ルイ オヨビ トコフェロール ルイ ノ イッセイ ブンセキ","subitem_title_language":"ja-Kana"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"赤坂, 和昭","creatorNameLang":"ja"},{"creatorName":"アカサカ, カズアキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1446","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Akasaka, Kazuaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1447","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-07-31"}],"displaytype":"detail","filename":"079-069-080.pdf","filesize":[{"value":"8.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"079-069-080","url":"https://shokei.repo.nii.ac.jp/record/504/files/079-069-080.pdf"},"version_id":"f67fabf5-427f-467b-a5a3-8d933da7b4f4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"逆相クロマトグラフィー","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"低温分離","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"トコフェロール","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"トコトリエノール","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"二次元HPLC法","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"reversed phase HPLC","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"separation at low temperature","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tocopherol","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tocotrienol","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"2-dimensional HPLC","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"逆相HPLC法によるトコトリエノール類及びトコフェロール類の一斉分析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"逆相HPLC法によるトコトリエノール類及びトコフェロール類の一斉分析","subitem_title_language":"ja"},{"subitem_title":"Simultaneous determination of tocotrienols and tocopherols by a reversed-phase HPLC","subitem_title_language":"en"}]},"item_type_id":"2","owner":"8","path":["49"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-07-31"},"publish_date":"2020-07-31","publish_status":"0","recid":"504","relation_version_is_last":true,"title":["逆相HPLC法によるトコトリエノール類及びトコフェロール類の一斉分析"],"weko_creator_id":"8","weko_shared_id":-1},"updated":"2024-05-30T01:50:58.617570+00:00"}